In contrast to chemical single parameter analysis, luminous bacteria tests are able to evaluate the toxicity of a sample. As an internationally standardised method (EN ISO 11348) it is one of the most important biological tests for toxicity analysis beside the also standardised fish-, daphnia- and algae-tests. For the performance of luminous bacteria tests a wide range of liquid- and freeze-dried products is available.
The inhibition of the bacteria’s bioluminescence is determined in a static test by mixing a defined amount of a sample with a luminous bacteria suspension in a cuvette. Subsequently, the inhibition of the luminescence in the sample is determined in comparison to an uninhibited control solution.
The evaluation of the toxicity analysis is performed with the BioFix® Lumi-10, a universal luminometer, which is suitable for portable use.