- NOA 280i - Nitric Oxide Analyzer

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Sievers - NOA 280i - Nitric Oxide Analyzer

The Sievers Nitric Oxide Analyzer (NOA 280i) offers the most versatile detection system for Nitric Oxide (NO) analysis. Using GE Analytical Instruments' highly sensitive, ozone-chemiluminescence technology, the NOA 280i has unsurpassed versatility for liquid and exhaled breath nitric oxide (NO) measurement. With over 700 publications using the Sievers Nitric Oxide Analyzer, the NOA 280i has proven to be the instrumentation of choice by researchers worldwide. The Sievers NOA can be used to measure liquid samples or exhaled breath samples of nitric oxide.

NOA Exhaled Breath Applications

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The Sievers Nitric Oxide Analyzer (NOA) 280i can be used to measure exhaled NO using a wide range of techniques including On-line, Off-Line, Nasal, Breath-by-Breath and Chamber Sampling. The NOA and its accessories permit measurements according to the American Thoracic Society recommendations and other research applications.

On-Line Sampling
Use our patented technique and the On-Line NO Breath Kit to measure on-line concentrations of NO in exhaled breath without nasal contamination. The NOA 280i's built-in exhaled pressure feedback system and optional Thermal Mass Flowmeter facilitate exhalation at constant flow rates from 10–1000 mL/s. The NOAnalysis REB program is used for flow meter calibration or restrictor selection, data collection and processing.

Off-Line Sampling
Our patented technique and Bag Collection and Sampling Kits permit collection of exhaled breath for later analysis. Two kits are available: Vital Capacity Kit for collection of exhaled breath at 350 mL/s (ATS recommendation) and the Deadspace Discard Kit that permits collection of exhaled breath under the same conditions as on-line measurements. The concentration of NO in the Mylar bags is stable for 8 to 12 hours and the bags can be reused after a simple cleaning procedure. The NOAnalysis Bag program is used for analysis of the samples.

Nasal Sampling
Use a constant transnasal flow of 3 L/min, with sidestream sampling and a slow oral exhalation against a resistance to measure nasal NO. The NOAnalysis REB program can be used for nasal measurements.

Breath-by-Breath Measurement
Use this technique to measure exhaled NO in subjects who cannot perform the on-line or off-line maneuvers (i.e., ventilated subjects, small children, animals) The NOA 280i's exhalation pressure transducer, and the NOAnalysis Breath program, determine the beginning and end of each exhalation and the concentration of NO for each breath.

Chamber Sampling
The concentration of exhaled NO in spontaneously breathing small animals can be measured using the Chamber Sampling method. Using head-out or whole body plethysmographs, permits exhaled breath to accumulate in the chamber for subsequent measurement by the NOA.

Other Gas Applications
Use the NOA 280i to measure gas-phase NO directly in the airways during bronchoscopy. The NOA can also be used to monitor NO production from other organs (eye, skin, gums).

NOA Liquid Applications

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The Sievers Nitric Oxide Analyzer (NOA 280i) and purge system measures nitric oxide and its reaction products, nitrite, nitrate and nitrosothiols, in virtually any biological fluid including: cell culture media, plasma, sera, urine, cerebral-spinal fluid, bronchial-alveolar lavage, perfusates, and tissue homogenates.

The Sievers Nitric Oxide Analyzer (NOA 280i) and purge system measures nitric oxide and its reaction products, nitrite, nitrate and nitrosothiols, in virtually any biological fluid including: cell culture media, plasma, sera, urine, cerebral-spinal fluid, bronchial-alveolar lavage, perfusates, and tissue homogenates.

Nitrate
Nitrate (NO3-), the major oxidation product of NO in some cell culture systems and most physiological fluids is formed when nitric oxide reacts with oxyhemoglobin or superoxide anion. Use the NOA 280i and purge vessel to measure total nitric oxide (nitrate + nitrite + nitrsothiols) using vanadium (III) chloride in hydrochloric acid at 90 °C (Braman, et al., Anal. Chem. 1989; 61: 2715-8).

Nitrite
Nitrite (NO2-), the major oxidation product of NO in the absence of oxyhemoglobin or superoxide anion, is formed when nitric oxide reacts with dissolved oxygen. Use the NOA 280i and purge vessel to measure nitrite using iodide and acetic acid at room temperature (Castegnaro, et al., Food Addit. Contam. 1978, 4:37-43).

Nitrosothiols
Nitrosothiols are present at low nanomolar concentrations and formed from the reaction of an NO species with thiols. The 280i, purge vessel and suitable reducing agent can be used to measure nitrosothiols in biological fluids.

Low molecular weight compounds such as S-nitrosoglutathione can be directly measured using a Cu(I)/Cysteine reagent. (Fang, et al., Biochem Biophys Res Comm 1999;252:535-540.)

Higher molecular species such as S-nitroso-albumin or S-nitroso-hemoglobin can be measured using iodide/iodine and acetic acid (Samouilov,et al., Analy Biochem 1998;258:322-330) after removal of nitrite by reaction with the Griess reagent or chromatographic separation. (Marley, et al., Free Rad Res 2000 :32(1):1-9. Gladwin, et al., PNAS 2000;97(18):9943-9948.)

Iron-Bound NO
Nitric oxide bound to iron in heme or other metal centers can be measured by combining the nitrosothiols measurement with and without treatment of the samples with KCN/K3Fe(CN)6. The difference is the amount of NO bound to the metal center (Gladwin, et al., PNAS 2000;97(21):11482-11487.

Iron-bound NO can be directly measured using a reagent containing acetic acid, KCN/K3Fe(CN)6, and sulfanilamide (Nagababu, et al., J Biol Chem 2003;278(47): 46349-56.

Sievers NOA Technical Literature

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Search in the Library for these and other resources for the Sievers Nitric Oxide Analyzer (NOA):

  • Application Notes and Papers
  • Brochures
  • Industry Links
  • Manuals

Options and Accessories

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Options and accessories for the Sievers NOA are grouped as follows:

Sievers NOAnalysis Software

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NOAnalysis is a suite of four programs (Liquid, REB, Bag and Breath) for collection and processing of data from the NOA. Data is collected via the computer serial port and the programs can be run on a PC (Win 95/98/NT/XP/Vista) or a Macintosh PowerPC (System 7 or higher). All files are saved in a tab-delimited text format for easy import into a spreadsheet or statistics program.

Liquid
The Liquid program is used with the purge vessel to acquire data, name the samples, integrate the peaks (using threshold and peak width detection and automatic select of start and stop integration marks), prepare calibration curves, calculate the concentration of the samples and save the results. New features in Version 3.21 include:

  • Create Sample Names Tables to identify samples prior to injection. Names can be added or deleted during data acquisition.
  • Manual Integration option — skips peak detection and allows setting start and stop integration marks
  • Change concentration units after calculation

Restricted Exhaled Breath (REB)
The REB program is for on-line measurement of exhaled NO. The program is used to acquire data, display feedback for the subject to insure constant flow exhalation, select NO plateaus using ATS criteria*, calculate the mean and average deviation from replicate measurements, and to reject outliers and save or print the results. New features in Version 3.21 include:

  • Use of either pressure or flow feedback for constant flow exhalation
  • New results printout including subject information, exhaled NO values, exhaled NO plots and optional image file for printing institution's logo
  • New results file format, measurements from different days can be saved in a single results file

Recommendations for Standardized Procedures for the Online and Offline Measurement of Exhaled Lower Respiratory Nitric Oxide and Nasal Nitric Oxide in Adults and Children-1999, Am J Respir Crit Care Med Vol 160. pp 2104?2117, 1999.

Bag
The Bag program is for the measurement of exhaled NO collected off-line in mylar bags. The program is used to acquire data, analyze NO levels in each bag, calculate averages for replicate samples from a subject, edit the number of samples per subject and save and print the results. During data acquisition, information about the sample can be entered into a table and NO levels set for each sample.

After data collection, outliers can be deleted and the results printed or saved in a tab-delimited text format for easy input into statistics or spreadsheet programs.

Breath
The Breath program is for breath-by-breath measurements of exhaled NO. Since this technique does not exclude nasal NO or provide for constant flow exhalations, it is only used for those subjects who cannot perform the on-line or off-line methods. The breath program is used to determine the time offset between the NO and pressure signals, acquire data, detect the beginning and end of each exhalation using the pressure signal, adjust the offset (if necessary) and calculate the maximum, minimum and end-tidal or plateau NO concentration for each breath along with overall mean and standard error. Outliers can be rejected and the results printed and saved.

Sievers NOA Specifications

Operating Specifications

Sensitivity: Gas: < 1 ppb, Liquid: ~1 picomole
Range: Gas: <1–500,000 ppb, Liquid: Nanomolar to millimolar
Response Time: Electronics: 67 milliseconds to 90% full scale Lag Time: 1 second
Repeatability: Gas: ± 5% Liquid: ± 5–10%
Sample Size: Gas: 10–300 mL/min Liquid: 0.001–5 mL
Display: Back-lit LCD screen ppb/ppm or mV
Outputs: Analog: 0–1V, 0–10 V Digital: RS-232 (9600-38.4K baud) Printer: Parallel port
Data Sampling Rate: 0.002–32 samples/second
Power Requirements: 120 V, 60 Hz (6A) 100 V, 50 or 60 Hz (7A) 230 V, 50 Hz (3A)
NOA: Height: 16 in (41 cm) Width: 6.2 in (16 cm) Length: 20 in (51 cm) Weight: 35 lbs (16 kg)
Vacuum Pump with Installed Trap: Height: 14.5 in (37 cm) Width: 7.5 in (19 cm) Length: 19 in (48 cm) Weight: 47 lbs (21.5 kg)
Certifications: Declaration of conformity

Operating Environment
 
Ambient Temperature: 32° to 86°F (0° to 30°C)
Relative Humidity: 0–90%
 
Fuse Requirements

Main Fuse: 100 VAC model: T, 10A, 250V 120 VAC model: T, 5A, 250V 230 VAC model: T, 5A, 250V
Ozone Fuse: 100 VAC model: T, 200mA, 250V 120 VAC model: T, 200mA, 250V 230 VAC model: T, 100mA, 250V

GE Analytical Instruments
GE Analytical Instruments
6060 Spine Road
Boulder ,  CO 80301

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