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capillary electrophoresis Applications

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    Determination of amino acids in beer and wort by capillary electrophoresis

    Free forms of amino acids in beer originate mostly from malt. The following method enables fast quantitative determination in beer and wort of the following free amino acids: arginine, lysine, tyrosine, phenylalanine, histidine, leucine and iso-leucine (total), methionine, valine, proline, alanine, glycine, cystine, tryptophan, aspartic and glutamic acids. Total content of amino acids is fdetermined after hydrolysis of proteins. 

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of protein purity and heterogeneity with capillary gel electrophoresis and capillary isoelectric focusing

    INTRODUCTION

    Antibodies, protein-based therapeutics, and other recombinant proteins are final products in biotechnological and pharmaceutical industry. Determination of their purity, stability, and hetero geneity is of utmost importance since post-translational modifications as well as degradation processes can change drastically the biological activity of these proteins.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of phenoxycarboxylic acid herbicides in water using high performance capillary electrophoresys method using Capel systems

    The method allows determination of herbicides classified as phenoxycarboxylic acids, specifically: 2,4-dichloro-- phenoxybutyric acid (2,4-DB), 2,4-dichlorophenoxypropionic acid (2,4-DP, Dichlorprop), 2,4-dichloropheno-- xyacetic acid (2,4-D), and phenoxyacetic acid in samples of natural, potable and treated waste water by capillary electrophoresis. 

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of hesperidin and naringin in juices using CAPEL-105M capillary electrophoresis system

    The method is intended for determination of hesperidin and naringin using capillary electrophoresis technique in order to reveal adulteration of juices. These components are tested in citrus juices, concentrated juices, nectars, juice-containing beverages, and fruit sauces that are produced primarily from oranges, grapefruits, tangerines, lemons, and limes. 

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determnation of albumin concentration in urine

    INTRODUCTION

    The capillary electrophoresis method provides det ermination of albumin concentration in urine.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of serum protein fractions

    INTRODUCTION

    The method allows determination of relative amou nts of albumin and globulins in human serum using capillary electrophoresis methods.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of inorganic cations in water samples

    Introduction

    The method allows determination of ammonium, lithium, sodium, potassium, magnesium, calcium, strontium and barium cations in samples of natural, potable, and waste water by the capillary electrophoresis method.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of hesperidin and naringin in juices

    Introduction

    The method is intended for determination of hesperidin and naringin using capillary electrophoresis technique in order to reveal adulteration of juices. These components are tested in citrus juices, concentrated juices, nectars, juice-containing beverages, and fruit sauces that are produced primarily from oranges, grapefruits, tangerines, lemons, and limes.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of Anions in Beverage

    Introduction

    The method is used for the determination of the mass concentration of chloride, sulfate, and nitrate in beverages by capillary electrophoresis. The method can be applied for all types of non-alcoholic and alcoholic beverages including sport and energetic drinks, juices, beer and beer products, wines, brandy and spirits, liquors, and vodka.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of 4-carboxybenzaldehyde & p-toluic acid in purified terephthalic acid according to ASTM D7881 & ASTM D7882 test methods

    Introduction

    These test methods cover the determination of 4-carboxybenzaldehyde (4-CBA, 4-formylbenzoic acid) and p-toluic acid (p-TOL, 4-methylbenzoic acid) in purified terephthalic acid (PTA) by capillary electrophoresis (CE) with normal or reverse voltage mode and UV detection.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of choline (vitamin B4) in feedstuffs, compound feeds, fodder additives, and feed raw materials

    Introduction

    The method is used for the determination of the mass fraction of free choline (synthetic and natural) in feedstuffs by capillary electrophoresis. The method can be applied for all types of feedstuffs, compound feeds, fodders, premixes, fodder additives, and feed raw materials. The present method does not allow the determination of bound choline.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of water-soluble forms of inorganic and organic anions in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments

    The method allows determination of water-soluble forms of inorganic (chlorides, sulfates, nitrates, fluorides and phosphates) and organics (acetates, formates, and oxalates) anions in soils, clays, peats, wastewater silts, activated sludges and bottom sediments by capillary electrophoresis method. 

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of dyes in beverages

    The method allows identification and determination of synthetic dyes in samples of soft and alcoholic drinks by capillary electrophoresis.

    The Capillary Electrophoresis (CE) method for the determination of synthetic dyes is based on their differential migration in a narrow quartz capillary under the influence of the applied electric field. Identification and quantitative determination of dyes is performed by measuring the UV absorbance at 254 nm (for “CAPEL® -103РТ/104Т” systems) or 215 nm (for “CAPEL® -105/105М” systems) wavelength. 

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of total sulfur dioxide in wine and beer

    Method allows determination of mass concentration of total sulfur dioxide (sulfurous acid and its salts, food additives E220–E228) in wine, wine materials, beer and beer products by capillary electrophoresis (CE) using capillary electrophoresis system “CAPEL® -105M”. The mass concentration of total sulfur dioxide is regulated by normative documents to be held within 100– 400 mg/L for different types of wine and wine materials and within 20–50 mg/L for beer products. According to European and Russian regulations it is allowed not to indicate on the labels the presence of sulfur dioxide in beverages if its concentration is less than 10 mg/L. 

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of organic acids in beverages

    The method allows determination of organic acids, in soft drinks (juices, fizz drinks) and alcoholic drinks (wines, beer). 

    The capillary electrophoresis method for evaluation of cations concentration is based on differential migration and separation of cations in electric field due to the difference in their electrophoretic mobility. Identification and quantitative determination of the analyzed cations is performed by indirect detection measuring UV absorption at 254 nm (for “CAPEL® -103RT/104T” systems) or 267 nm (for “CAPEL® -105/105M” systems) wavelength.

    The determination is not hindered by the presence of anions of ammonia, lithium, strontuim, barium, manganese, ferrum (II) in the quantities that are typical for the analyzed drinks. 

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Determination of the carbendazim content in orange and other citrus juice produce

    Introduction

    Fungicide Carbendazim (Mercazole, Carbendazole) is widely used in agriculture to exterminate black mold (spot) on orange trees and treat fungus diseases of other fruit-bearing plants. According to the data of the World Health Organization, Carbendazim is toxic for liver, impacts reproductive system, and is considered to be a potentially cancerogenic compound. In most of the developed countries, the residual content of this fungicide is subject to national and international regulations. Researchers of LUMEX INSTRUMENTS, Ltd. have developed an analytical procedure for determination of the Carbendazim content in orange and other citrus juice produce using a capillary electrophoresis system CAPEL-105М.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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