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sample preparation process Applications

  • Improved Reproducibility and Reduced Sample Preparation Time for the HPLC Analysis of Aflatoxins in Raw Peanut Paste

    The US FDA and international regulatory agencies have set contamination levels for aflatoxins in animal feedstuffs. Since Aspergillus may infect commodities pre-harvest, during storage or during processing, monitoring for aflatoxins in associated agricultural commodities at all stages of production is requisite. Field screening methods exist that are adequate to estimate contamination levels for aflatoxins. When additional confirmation or quantification is desired, chromatographic laboratory analysis is often necessary. Preparation of matrix samples prior to chromatographic analysis typically requires extraction and purification. Commonly, immunoaffinity columns (IAC), which employ a multi-step bind and elute mechanism to concentrate and purify aflatoxins, are used to purify matrix samples for subsequent analysis. Solid phase extraction (SPE), an alternate method which may use interference removal, can also be employed.

    By MilliporeSigma based in Bellefonte, PENNSYLVANIA (USA).

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    RAPID ANALYSIS OF TOTAL MERCURY IN WASTE WATERS AND PROCESS WATERS WITH ELEVATED MERCURY CONTENT USING DIRECT PYROLYSIS TECHNIQUE

    A conventional standard method of total mercury determination in water using atomic absorption spectrometry (AAS) involves preliminary sample digestion that takes from 30 minutes to 8 hours depending on the digestion conditions. The use of a mercury analyzer RA-915M/RA-915+ with Zeeman background correction in combination with PYRO-915+ pyrolysis attachment provides direct determination of mercury in process and waste waters contaminated with mercury without digestion or any other sample preparation stages. The measuring method is based on thermal atomization of mercury from a sample using a PYRO-915+ attachment and its consequent determination by flameless AAS with Zeeman background correction using the RA-915M mercury analyzer. The total time needed for determination of mercury is not longer than 2 minutes. RA-915+/915M is compliant with US EPA 7473 and ASTM D7622 methods.

    By Lumex Instruments Group based in Mission, BRITISH COLUMBIA (CANADA).

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    Chromatography laboratory instrumentation for environmental analysis

    Determining pollutants in environmental and food matrices requires efficient sample preparation and analytics. BUCHI provide a wide selection of solutions for reliable, flexible and safe sample processing procedures in the laboratory work flow for environmental analysis.

    By B√úCHI Labortechnik AG based in Postfach, SWITZERLAND.

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    EDXRF spectrometers for metal & ore

    Quality control of the various metal industry processes. Metal forms an essential material in modern manufacturing, metal products being used everywhere. Using ED-XRF allows to measure a wide range of elements and concentrations - C(6)-Fm(100) - without the need for sample preparations (heating or destroying the sample). Samples can be measured in the form of loose powders or pressed into pellets and ready for measurement within seconds.

    By Xenemetrix Ltd. based in Migdal Haemek, ISRAEL.

  • Automated System for High Throughput PCR Setup

    Modern genomics labs are looking for high-throughput, quality-data workstations to perform PCR reactions at a reduced cost. PCR reaction setup is a tedious and time consuming process as it requires mixing multiple reagents in specific ratios to achieve the proper master mix for amplification. Moreover, the exact volumes of the reagents and buffers depend upon the total number of samples to be amplified and the desired final concentration of sample DNA. Automation of such procedures eliminates both human error and contamination problems generally associated with manual process. To provide an automated solution to such a cumbersome protocol, Aurora Biomed’s VERSA 110 PCR Setup Workstation was developed for the preparation of reagents and samples used in an amplification protocol in 96-well format.

    By Aurora Biomed Inc. based in Vancouver, BRITISH COLUMBIA (CANADA).

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