John Wiley & Sons, Ltd.

A benchmarking method to measure dietary absorption efficiency of chemicals by fish

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Understanding the dietary absorption efficiency (ED) of chemicals in the gastro‐intestinal (GI) tract of fish is important from both a scientific and a regulatory point of view. However, reported fish ED for well‐studied chemicals are highly variable. In the present study, we developed and exploited an internal chemical benchmarking method that has potential to reduce uncertainty and variability, and thus improve the precision of measurements of fish ED. We applied our benchmarking method to measure the gross ED for 15 chemicals with a wide range of physicochemical properties and structures. 2,2',5,6'‐tetrachlorobiphenyl (PCB53) and decabromodiphenyl ethane (DBDPE) were selected as absorbable and non‐absorbable benchmarks, respectively. Quantities of chemicals determined in fish were benchmarked to the fraction of PCB53 recovered in fish, and quantities of chemicals determined in feces were benchmarked to the fraction of DBDPE recovered in feces. The performance of the benchmarking procedure was evaluated based on the recovery of the test chemicals and precision of ED from the repeated tests. Benchmarking did not improve precision of the measurements, however, after benchmarking, the median recovery for 15 chemicals was 106%, and variability of recoveries was reduced as compared to before benchmarking, suggesting that benchmarking could account for incomplete extraction of chemical in fish and incomplete collection of feces from different tests. Environ Toxicol Chem © 2013 SETAC

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