The performance of two bench scale activated sludge reactors with two feeding regimes, continuous fed (an up-flow aerobic/anoxic sludge fixed film (UAASFF) bioreactor) and batch fed (sequencing batch reactor (SBR)) with intermittent aeration, were evaluated for simultaneous nutrients (N, P) removal. Three significant variables (retention/reaction time, chemical oxygen demand (COD): N (nitrogen): P (phosphorus) ratio and aeration time) were selected for modeling, analyzing, and optimizing the process. At high retention time (≥6 h), two bioreactors showed comparable removal efficiencies, but at lower hydraulic retention time, the UAASFF bioreactor showed a better performance with higher nutrient removal efficiency than the SBR. The experimental results indicated that the total Kjeldahl nitrogen removal efficiency in the UAASFF increased from 70.84% to 79.2% when compared to SBR. It was also found that the COD removal efficiencies of both processes were over 87%, and total nitrogen and total phosphorus removal efficiencies were 79.2% and 72.98% in UAASFF, and 71.2% and 68.9% in SBR, respectively.