A Feasibility Study for Assessment of In-situ Bioremediation Potential of a Crude Oil Degrading Pseudomonas Consortium

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Following is a link and abstract for an article published in the Journal for Scientific Research wherein a Pseudomonas consortium isolated from a site contaminated with crude oil was applied and effectively used to remediate the same contaminated site under particular environmental conditions. 

 

Link to pdf:  http://www.banglajol.info/index.php/JSR/article/view/2601/3361

 

A Feasibility Study for Assessment of In-situ Bioremediation Potential of a Crude Oil Degrading Pseudomonas Consortium

A. Mittal, P. Singh

Abstract

A microcosm study evaluating inoculums addition of mixed bacterial consortium to stimulate in-situ bioremediation of crude oil contaminated soil was conducted. In feasibility study, out of five treatments the application of bacterial consortium, nutrients and environmental factors resulted in 79.16% removal of TPH in 60 days, compared to 30.24% removal of TPH carried out by indigenous microflora. Gas chromatograms of original spilled oil and bioremediated oil by the addition of developed consortium shows that Pr/Ph ratio decreased progressively from 2.358 to 1.626. The results showed that the ratio of di/tri aromatics decreased from initial 0.63 to 0.25 with progressive treatment of nutrient addition, as nutrient + tilling, nutrient + tilling + microbial seeding. Similar effect was observed in di/di + tri aromatics ratios which also decreased 0.31 to 0.20 by bioaugmentation only.

 

Keywords: Hydrocarbons; Pseudomonas; Biodegradation; Gas Chromatogram.

 

© 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

 

DOI: 10.3329/jsr.v2i1.2601                 J. Sci. Res. 2 (1), 127-137 (2010)

 

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