John Wiley & Sons, Ltd.

A simple method to determine mineralization of 14C‐labeled compounds in soil

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Degradation of organic compounds in soil is often determined by measuring decrease of parent compound and analyzing occurrence of its metabolites. However, determination of carbon species as end products of parent compound dissipation requires the use of labeled materials which allow more accurate determination of the environmental fate of the compound of interest. The current conventional closed system widely used to monitor degradation of 14C‐labeled compounds in soil is complex and expensive as well as requiring a specialized apparatus and facility. In the present study, we describe a simple system that facilitates measurement of mineralization of 14C‐labeled compounds applied to soil samples. In the system, soda lime pellets to trap mineralized 14C‐carbon species including carbon dioxide (CO2) were placed in a cup, which was then inserted above the treated soil sample in a tube. Mineralization of [14C]2,4‐D applied to soil samples in the simple system was compared to that in the conventional system, and the simple system provided an equivalent detection of 14C‐carbon species mineralized from the parent compound. Our results demonstrate that this cost‐ and space‐effective simple system is suitable for examining degradation and mineralization of 14C‐labeled compounds in soil and further could potentially be used to investigate their mineralization in other biological matrices. Environ Toxicol Chem © 2014 SETAC

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