Alternative Methods to RSK 175 Using Purge and Trap Concentration and Automated Headspace for the Analysis of Dissolved Gases in Drinking Water
Police and forensic laboratories around the world routinely use headspace-gas chromatography for volatile components in biological fluids. One of the more common headspace analyses is the determination of blood alcohol in cases involving suspected DUI or OVI. The blood alcohol limit for some countries, like Korea and South Africa, is as low as 0.05%.
This study evaluates Teledyne Tekmar’s HT3 automated headspace vial sampler for the analysis of blood alcohol using the new YL6500 Gas Chromatograph from Young Lin Instrument Co., Ltd.
The volatile compounds presented in this paper include acetaldehyde and acetone, metabolized components of ethanol in the blood, methanol, a potential contaminant of ethanol, and isopropanol, which can be absorbed or inhaled from rubbing alcohol vapors. The compounds are quantified using external and internal standard methods. Either n-propanol or tert-butanol was used as internal standards.
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