Analysis of Atrazine Metabolites Using Ascentis™ C18

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Courtesy of Sigma-Aldrich Co., LLC

This application demonstrates the suitability of the Ascentis C18 for the analysis of atrazine metabolites. An Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles was used to separate the compounds.  A 85:15, 10 mM ammonium acetate (pH 5.0 with acetic acid):acetonitrile mobile phase was used at 1.0 mL/min.  All samples where diluted to 20 μg/mL in 56:44, buffer:methanol.  Detection was UV at 254 nm.  Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.

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