Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS


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Nitrofuran antibiotics are veterinary drugs. The use of these in food production is already banned in the United States,
Australia, Canada, Japan, Singapore, Bangladesh, and the European Union because of a possible increased cancer-risk
through long-term consumption.

Trace amounts of nitrofurans contamination has been found in warm-water prawns, shrimp, and chicken. The
contamination may occur from deliberate, direct misuse of the drugs as well as from contaminated feed.

The analyses of nitrofurans are based on the detection of the metabolites of the parent drugs. Since the parent drugs are
very rapidly metabolized and the tissue-bound metabolites are detectable for several weeks after administration, the
metabolites are a much better marker for the detection of the abuse of these antibiotics.

The metabolites of furazolidone, furaltadone, nitrofurantoine and nitrofurazone are 3-amino-2-oxazolidinone (AOZ),
3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AH), and semicarbazide (SC), respectively.Nitrobenzaldehyde, the most commonly used derivatizing reagent for these metabolites, forms a nitrophenyl derivative for LC/MS analyses.

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