Analysis of PCB in water using the envirogard test system

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MATRIX: Water

ANALYTE: PCB

LIMIT OF DETECTION:
Aroclor 1248: 1 ppm
Aroclor 1260: 2 ppm
Aroclor 1242: 2 ppm
Aroclor 1254: 1.1 ppm

BACKGROUND:
This application describes a field screening method for determining PCB concentrations in water. A chemical reaction is induced that causes a color change indicative of PCB in the sample. The PCB concentration is estimated from the absorbance at 450 nanometers (nm) on a differential photometer.

PRECAUTIONS:
Extraction and analysis should be performed in a well ventilated area. Laboratory technicians should wear chemical resistant gloves and safety glasses. Use gloves during the entire procedure..

MATERIALS:
Envirogard PCB Test Kit. Methanol (ACS Reagent Grade). Deionized or distilled water. Equipment: Envirogard Field System (with photometer).

PROCEDURE:
1. Follow the procedure in the Envirogard PCB in Soil Test Kit instructions to analyse samples (ignoring the soil extraction steps).
2. Add 25 uL of water sample (instead of 25 uL of soil extract) to each corresponding antibody tube (which contains 500 uL of buffer as specified in Step 1). In addition, add 25 uL of clean methanol to each sample tube in order to compensate for the methanol normally present in a soil extract and in the prepared standards.
3. Add 25 uL of deionized water to each calibrator/negative control antibody tube (which contains 500 uL of buffer as specified in Step 1). Continue with preparation of the kit standards and negative control per normal kit instructions and proceed with the remainder of the test as written.

INTERPRETATION:
Upon completion of the assay the water samples will have the same detection levels as those given for the soil test. Interpretation varies depending upon calibrators analyzed. (For example, the 1 ppm calibrator for soil will generate < or > 1 ppm for water.)

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