For a determined substrate concentration, the most significant environmental factors are temperature, pH and oxygen availability. The well known critical influence of those factors on the nitrification are here analyzed by making use of a specific software and advanced respirometry based on a dynamic LFS system, where the test conditions can be set in the corresponding test-settings board and their levels modified throughout the test performance. In this way, it is possible to get and asses the trajectory of the dynamic exogenous respiration rate coming from the ammonia removal for different levels of temperature, pH and oxygen. Then, from each respiration rate value the nitrification rate and nitrification capacity are determined.
For a better understanding, the present study is conducted by means the summary of three different real cases of study related with the nitrification process for each of the those critical factors. On each of the cases, by making use of the advanced software, we can get the correspondent respirograms and results, to be precisely analyzed and assessed.