Application of an optimized system for the well-defined exposure of human lung cells to trichloramine and indoor pool air

Sep. 1, 2011 - By: C. Schmalz , H. G. Wunderlich , R. Heinze , F. H. Frimmel , C. Zwiener , T. Grummt

In this study an in vitro exposure test to investigate toxicological effects of the volatile disinfection by-product trichloramine and of real indoor pool air was established. For this purpose a set-up to generate a well-defined, clean gas stream of trichloramine was combined with biotests. Human alveolar epithelial lung cells of the cell line A-549 were exposed in a CULTEX® device with trichloramine concentrations between 0.1 and 40 mg/m3 for 1 h. As toxicological endpoints the cell viability and the inflammatory response by the cytokines IL-6 and IL-8 were investigated. A decreasing cell viability could be observed with increasing trichloramine concentration. An increase of IL-8 release could be determined at trichloramine concentrations higher than 10 mg/m3 and an increase of IL-6 release at concentrations of 20 mg/m3. Investigations of indoor swimming pool air showed similar inflammatory effects to the lung cells although the air concentrations of trichloramine of 0.17 and 0.19 mg/m3 were much lower compared with the laboratory experiments with trichloramine as the only contaminant. Therefore it is assumed that a mixture of trichloramine and other disinfection by-products in the air of indoor pool settings contribute to that effect.

Keywords: cell viability, CULTEX®, cytokines IL-6 and IL-8, human lung cells exposure, indoor pool air, trichloramine

Most popular related searches
- disinfection by-product indoor air swimming pool air in vitro swimming pool exposure testing exposure test toxicology swimming pool indoor air disinfection product

Customer comments

No comments were found for Application of an optimized system for the well-defined exposure of human lung cells to trichloramine and indoor pool air. Be the first to comment!

Contact

Loading... Drop file here or browse Great, file uploaded. Change File Drop file here or browse

Yes, please send to similar suppliers.

Application of an optimized system for the well-defined exposure of human lung cells to trichloramine and indoor pool air

Most popular related searches

Related articles

Customer comments