Keywords: yeast, S cerevisiae, fingerprint, DNA technology, RAPD, marker and genetic biodiversity
Application of DNA technology to study the genetic biodiversity of yeast strains (S. cerevisiae) based on RAPD markers
A genomic DNA of nine yeast strains has been extracted from total cellular DNA, previously amplified according to RAPD procedure. Two random 10-mer primers were used to assay polymorphisms among nine yeast strains: Candida albicans, Candida tropicals, Rhodotuala glutinis, Saccharomyces cerevisiae GT160-34B, positive control (standard DNA) yeast, S. cerevisiae GT160-346 Mutant, S. cerevisiae strain obtained from the Egyptian Sugar and Distillation Company, Hawamdia, a diploid wild type S. cerevisiae and a haploid wild type S. cerevisiae. Different RAPD fragment patterns were observed for different nine yeast strains. Results showed that there are great differences among all nine strains. Also, data demonstrated that the RAPD marker is useful for systematic investigation at the level of yeast strains.