Assessment of the osteoblastic cell response to a zinc glass reinforced hydroxyapatite composite (Zn-GRHA)
Hydroxyapatite (HA), Ca10(PO4)6(OH)2 and tricalcium phosphate (TCP) bioceramics have been used as graft materials. However, optimal biological performance has not been established yet and zinc, being a biosafe, biocompatible element, could favour for specific osteoblastic cell response. Therefore, this paper investigates the preliminary results and potential impact of zinc glass reinforced hydroxyapatite (Zn-GRHA) on a human osteoblastic cell system. The biological behaviour of Zn-GRHA samples was assessed by confocal laser scanning microscopy, while material characterisation was performed by SEM-EDX and XRD analysis. Established cultures reported an increased proliferation and a confluent cell layer in some areas of the material surface at day two. Cells were spread all over the material surface and established multiple cell-to-cell interactions relying on prominent cytoplasmic processes. At day six, confluent cell layers were verified on the Zn-GRHA material's surface, reporting an improved biological response, compared to control (hydroxyapatite).
Keywords: zinc glass-reinforced hydroxyapatite, Zn-GRHA composites, cell proliferation, osteoblastic cell response, bioceramics, biomaterials, graft materials