John Wiley & Sons, Ltd.

AuNP‐based colorimetric aptasensor for rapid detection of six organophosphorus pesticides


Fast immunoassay‐based screening methods are unavailable for most of small‐molecule pesticides due to lack of immunogenicity and the difficulty in obtaining antibodies by animal immunization. Aptamers are single‐stranded DNAs selected through an in vitro process, which can bind to any target including non‐immunogenic small molecules with high affinity and specificity. Though various aptamer‐based sensing methods have been developed for antibiotics, microorganisms, heavy metal ions, biotoxins, etc, there are few reports on aptamer‐based methods for quick detection of organophosphorus pesticides. The gold nanoparticle (AuNP) colorimetric assay is a widely utilized rapid detection method because of its properties such as easy operations and visualized results. In this study, organophosphorus pesticide aptamers were adsorbed on the surface of AuNPs to stabilize the AuNP solution against high concentrations of salt to prevent AuNP aggregation. Upon addition of targets, the aptamers binding to the targets are detached from the AuNPs, resulting in subsequent aggregation of AuNPs and a color change from red to purple‐blue. The proposed method can detect six organophosphorus pesticides with good recoveries from 72% to 135% in environmental river water samples. Our study provides a new way for simple, rapid and multiplex detection of organophosphorus pesticides. This article is protected by copyright. All rights reserved

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