AutoMate-Q40 Automated QuEChERS Extraction for Pesticide Residues in Botanicals
Abstract
QuEChERS is a Quick-Easy-CheapEffective-Rugged-Safe extraction method that has been developed for the determination of pesticide residues in agricultural commodities. Since its development, QuEChERS has been applied to many different classes of commodities ranging from fruits and vegetables to dry grains and teas. In 2007, the United States Food and Drug Administration (USFDA) announced that the makers of dietary supplements (botanicals) must adhere to stricter regulations.1 Since a majority of the dietary supplements are sourced from botanicals, it called for the determination of pesticide residues in samples. It was determined that the QuEChERS extraction was suitable to extract pesticide residues from the botanical samples.
This study will evaluate the performance and versatility of the AutoMate-Q40, an automated QuEChERS extraction platform. Liquid Chromatography coupled to a triple-quadrupole Mass Spectrometer (LCMS/MS) was used to determine pesticide residues from the botanical samples. Quantification was based on matrix-matched calibration curves with the use of internal standard to ensure method accuracy. QC samples were evaluated at various levels to ensure precision and accuracy of the AutoMate-Q40 extraction.
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