Bacterial synthesis of nanostructured hydroxyapatite using Serratia marcescens PTCC 1187
Nanostructured hydroxyapatite (nHA) exhibits improved mechanical properties and biocompatibility. To optimise the benefits of nano-sized precursors, the particles must have a uniform shape and size and minimum degree of agglomeration. In this study nHA was synthesised using an Iranian strain of Serratia. The powder was synthesised in different pH of reaction solution and incubation temperatures. After calcinations treatment in different temperatures, the powder was characterised using different analytical methods. Results showed that the optimum powder production was achieved at the pH around eight and temperature of 37°C. The powder particles were single crystal with the size of 25-30 nm. Moreover the shape and the size of the particles were relatively uniform and the amount of agglomeration was less compared to conventional methods. This powder could be used in regeneration of bone defects, fabrication of medical implants and as a vector for pharmaceuticals and biological materials such as genes.
Keywords: hydroxyapatite, phospahatse, serratia, biomineralisation, nanobiotechnology, nanotechnology, Iran, nanostructures