Best practices for cell culture confluency calculation with InCellis
Cells are used as a working tool everyday in all R&D labs and quality checks must be performed in order not to compromise downstream experiments. In cell culture applications, confluence assessment is an important parameter that is commonly used to estimate the proportion of adherent cells. Most confluency measurements are estimations of the area covered by the cells on a growth surface. The common method to determine cell culture confluency is visual estimation but it is not consistent or accurate enough for scientific experiments. As an example, most of transfection reagents came with a specific confluence at the time of transfection.