Best practices for tough sample grinding in molecular biology using Precellys
The biggest challenge when working with fibrous, elastic and tough samples is efficient sample processing. The presence of elastic tissue, calcium phosphate (a rock-hard mineral) and other minerals can develop into a solid and tough matrix which can sometimes be difficult to homogenize.
Moreover, heat generation inside the lysing kit tubes during the process results in degradation and denaturation of these molecules such as proteins and RNA. Heat generation is even more severe when working with harder samples that require higher energy for efficient homogenization, resulting in a more challenging process.
The Precellys Evolution homogenizer is capable of grinding hard samples within minutes thanks to its 3D-bead beating technology. When paired with the new Cryolys Evolution cooling unit, it also prevents heat generation inside the lysing kit tubes during the process, thus avoiding degradation and denaturation of thermo-sensitive molecules.