John Wiley & Sons, Ltd.

Bioaccumulation, subcellular distribution and acute effects of chromium in Japanese medaka Oryzias latipes

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Chromium (Cr) is an essential element but toxic at elevated concentrations to aquatic organisms. In the present study, adult Japanese medaka (Oryzias latipes) were exposed to a sublethal hexavalent chromium (Cr(VI)) concentration via dissolved and dietary exposures for 6 d. We measured Cr bioaccumulation in different tissues, subcellular distribution in the liver, effects on antioxidants, acetylcholinesterase (AChE), and Cr‐induced lipid peroxidation. Our results showed that bioaccumulation increased dramatically in all tested tissues from dissolved exposure but only significantly in the intestine from dietary treatment, implying dissolved exposure may be predominant for Cr accumulation in medaka. Subcellular distribution revealed Cr accumulated in the liver was mainly (46%) associated with the heat‐stable proteins (HSP) fraction. Among all the antioxidants examined, catalase (CAT) responded to dissolved Cr‐exposure in most tissues while superoxide dismutase (SOD) was less responsive. Meanwhile, malondialdehyde (MDA) concentrations were elevated significantly in most tissues examined in the dissolved Cr‐exposed fish, but only elevated in the liver and intestine in the dietary Cr‐exposed fish. The AChE activity in the brain was stimulated by 49% in the dissolved Cr‐exposed fish. Furthermore, reductions of condition factor (CF) and gonadosomatic index (GSI) were observed. In conclusion, our data help better understand Cr tissue distribution and acute effects of Cr in Japanese medaka. This article is protected by copyright. All rights reserved

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