John Wiley & Sons, Ltd.

Biochemical and morphological responses in chironomus riparius (Diptera, Chironomidae) larvae exposed to lead‐spiked sediment

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The aim of the present study was to assess the potential use of biochemical markers and mentum deformities as indicators of long‐term exposure to lead (Pb) in Chironomus riparius larvae. To do this, we measured three biochemical markers (i.e malondialdehyde level, metallothionein concentration and energy reserve content) as well as larval growth and mentum deformities after 16 days exposure to sediment containing Pb. The concentrations studied ranged from 3.5 to 505.5 mg/kg dw. Despite the bioaccumulation of Pb in Chironomus riparius bodies, frequencies of both mentum deformities and the dry weight were not significantly different between control and stressed groups. On the contrary, Pb exposure caused a significant increase of both malondialdehyde level and metallothionein concentration. The increase of body Pb concentrations did not significantly modify body copper (Cu) and zinc (Zn) concentrations. Moreover, we observed a decrease of total lipid content and an increase of glycogen content as a function of a dose‐response relationship, while no variation in protein concentrations was observed. Despite the adversaly effects observed at the biochemical level, the larval development was not affected. These results suggested that the measurements of MDA level, MT concentration and energy reserve content can be used as relevant biomarkers of long‐term sub‐lethal exposure to Pb in Chironomus riparius larvae. Environ Toxicol Chem © 2013 SETAC

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