Bioremediation, using CL-out microbes, clears the obstacles to Brownfield redevelopment by reducing wells contaminant concentrations in groundwater and removing the environmental stigma from the property. The CL-out bacteria were selected for their ability to degrade a wide range of organic chemicals under aerobic conditions. CL-out bioremediation of chlorinated solvents does not produce harmful breakdown products such as vinyl chloride.
CL-out is comprised of a patented microbial species, which were isolated from a contaminated site in northern United States. These species have the ability to reduce chlorinated organic contaminates, such as tetrachloroethylene (PCE) and trichloroethene (TCE) under aerobic conditions. Breakdown of PCE under aerobic conditions has been previously thought impossible to achieve, but lab studies and field applications have proven differently. PCE and TCE are broken down completely under aerobic conditions, without harmful byproducts. The theoretical pathway for chemical break down is based on the anaerobic pathways that have already been established. CL-out oxidizes chlorinated chemicals using a cometabolic metabolism, which requires the addition of dextrose to trigger the reactions. A dehalogenating enzyme first removes one chlorine atom from PCE, which has four chlorine atoms total, to produce TCE. This allows oxygenases enzymes, probably monooxygenases (MMO), to oxidize TCE forming an epoxide, which is further oxidized to 1,2-dihydroxy-TCE. Then 1,2-dihydroxy-TCE is oxidized completely to carbon dioxide, fatty acids, and water. CL-out is produced in a freezed-dried or liquid form. Once the freeze-dried CL-out is hydrated, then applied under light pressure through one-inch injection wells (vertical or horizontal), within 24 hours of adding water to the bag. The liquid form is ready-to-use.