John Wiley & Sons, Ltd.

Cadmium inhibits the vitellogenesis of freshwater crab Sinopotamon henanense

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Cadmium (Cd) may pose risks to freshwater organisms, including crabs that live at the interface of sediments and water column all year round. One of the major changes that occur during oocyte maturation of crabs is the production of vitellin (Vn). In the present study, we investigated the effects of Cd on oocyte size, Vn level, and vitellogenin (Vg) mRNA expression in the ovary of the freshwater crab Sinopotamon henanense. We studied the impacts of Cd on carbohydrate as well as on the protein metabolism, metallothionein (MT), glutathione (GSH) synthesis, energy‐related parameters and mRNA expression of genes involved in energy metabolism. After Cd treatment, Vn concentration, Vg mRNA expression and oocyte diameter decreased. There was less carbohydrate and enhanced protein catabolism in the ovary. Adenosine triphosphate/adenosine diphosphate (ATP/ADP) ratios, reduced/oxidized nicotinamide adenine dinucleotide (NADH/NAD+) ratios, mitochondrial membrane potential (△ψm) and energy production related mRNA expressions increased in the ovary after 10 days of Cd treatment and decreased after 20 days. MT and GSH synthesis were up‐regulated after 10 days upon Cd exposure. Moreover, Cd caused a time‐dependend up‐regulation of malondialdehyde. Our findings showed significant effects of Cd on vitellogenesis, which suggest that Cd slows down vitellogenesis in S. henanense due to excessive energy consumption and an activated defense system. This article is protected by copyright. All rights reserved

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