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Polycarbonate Membrane Filters for the Seq-Well Platform
The Seq-Well protocol uses PCTE membranes in an innovative platform for rapid single-cell transcriptomics. This powerful tool in the world of clinical discovery offers a precise snapshot of cellular behavior. As the product of a joint research venture between the Shalek and Love groups at MIT, this portable device combines single-cell sequencing with microfluidics technology. The system enables researchers to study RNA transcripts present in numerous individual cells at a given point in time. Thousands of...
Silver Membranes in Monitoring Respirable Crystalline Silica
Crystalline silica, most commonly found in the form of quartz, is a basic component of the earth; it’s found in soil, sand granite, and other minerals. During many industrial processes, crystalline silica is released as particles that are 100 times smaller than beach sand.1 Due to their size, these mineral particles cannot easily be cleared by human lungs. Instead, they persist in the respiratory system and form scar tissue, contributing to serious health problems for those experiencing prolonged exposure....
Transparent Polyester Membrane Filters Enable High Resolution Microscopy
Sterlitech now offers transparent polyester track-etch (PETE) membrane filters with sufficient clarity to perform high resolution optical imaging through the membrane. The key attribute of this material is a special, low density pore structure that mitigates light diffusion and renders the filters highly translucent. Furthermore, the membrane has very uniform thickness and flat surfaces so tissue cultures lie in one focal plane. Our transparent PETE filters are available in the 0.4 µm pore size rating...
eDNA Article Update
In a recent article, we described how environmental DNA (eDNA) has been used to detect the presence of aquatic species in lakes and rivers. eDNA studies have demonstrated great potential for surveillance of rare, endangered, and invasive species by simply collecting and analyzing water samples from target habitats. Dr. Caren Goldberg, of Washington State University, is one researcher using a unique combination of Sterlitech filters in her investigations of Yangtze giant softshell turtles in southeast Asia,...
Tech Tips: Membrane Preconditioning
What is membrane preconditioning and why is it recommended to precondition new flat sheet or spiral woundmembranes prior to use? Membrane preconditioning is a filtration step using deionized water as feed solution and is typically performed at a pressure equal to or higher than the anticipated testing pressure. During this process membrane pores are wetted and the membrane structure may go through compaction or swelling, affecting both the permeate flux and the rejection values. Preconditioning will help ensure...