Reduction of NOx- and particle emissions of Diesel engines is worldwide an important challenge for the engineers. Some unregulated components, like NO2, NH3 and naoparticles NP came in the focus of attention in the last years.Application of EGR and / or SCR can lower NOx, using RME (Bxx) can increase NOx. What happens with NP, parameter which enters in some further steps of exhaust gas legislation in Europe? The present paper informs about the results with EGR, B100 and SCR obtained on a medium duty Diesel engine in the versions: Euro 3 (w/o EGR) & Euro 4 (with EGR), both without particle filter. The investigations were performed according to the procedures of the international network project VERT dePN (de-activation, de-contamination, disposal of particles & NOx).The most important findings are:• the EGR of the new engine version E4 is active at middle load,• the NOx reduction potentials in ETC with combinations of the investigated measures are: EGR reduces NOx approx. in the same range, as B100 increases it (17-20%); SCR is the strongest reduction measure in the range of 73%. These potentials are similar at middle-load stationary operation. • the influences on nanoparticles counts emissions (PC) depend on different factors and can partly change between stationary and dynamic operation and with the use of B100.In summary: EGR and SCR can efficiently reduce NOx and overcompensate the effect of B100. EGR is most advantageous at low load, when SCR is not active.