Comparative Study of Operation Performance and Microbial Community between Chembioflocculation (CBF) Process and Chemically Enhanced Primary Treatment (CEPT) Process

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Chem-bioflocculation (CBF) process was a new municipal wastewater treatment process developed in recent years. The flocculant dosage was one of the most important factors in operation optimization of chem-bioflocculation(CBF) treatment process. Five levels of flocculant dosage (PAFC) from 30mg/L to 100mg/L were applied to compare operational performance between CBF process and chemically enhanced primary treatment (CEPT) process. Superior column loadings of COD, SCOD, SS, and NH4+-N were obtained in CBF process. The column loading of TP was present as close level in two processes. The biodiversities of microbial communities, including Bacteria, Acitnomycetes, Acidobacterium, and ammonia oxidizing bacteria (AOB), were analyzed by using PCR-DGGE method with group-specific primers at different flocculant dosage of polyaluminium ferric chloride (PAFC). The results indicated that CBF reactor contained special bacterial community structure other than CEPT reactor. The special distribution of bacterial population was even at different positions of two reactors. To quantify Bacteria cells, Real-Time PCR method was applied by using primers for 16SrDNA V3 regions. CBF reactor had a little less population size of Bacteria than traditional AS process, but great higher than that of CEPT reactor.

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