Inderscience Publishers

Covalently-bound organic monolayers for biosensor applications

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In this paper, we present a detailed investigation on monolayer formation with thicknesses amounting 1 nm to 3 nm on silicon and silicon nitride surfaces. Thermal and photochemical methods are successfully used to form such stable and densely backed monolayers. Further, functionalisation of these surfaces with activated esters, amine groups are also presented. Static water contact angle measurements, atomic force microscopy (AFM), infrared reflection absorption spectroscopy (IRRAS) and x-ray photoelectron spectroscopy (XPS) are used to follow the progress of the reaction and final characterisation of the resulting surfaces. These monolayers can be further functionalised to link DNA and other biomolecules.

Keywords: silicon surfaces, organic monolayers, photochemicals, biosensors, DNA, X-ray photoelectron spectroscopy, XPS, infrared reflection absorption spectroscopy, IRRAS, atomic force microscopy, AFM, monolayer formation, silicon nitride, biomaterials, nanomaterials

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