Cyanobacterial Toxins Detected by Thamnotoxkit (a Double Blind Experiment)
The Thamnotoxkit FTM was evaluated for detecting cyanobacterial toxins compared with the rat hepatocyte test and mouse test. This test kit is a 24-hour biosassay using larvae of the freshwater anostracan crustaceanThamnocephalus platyurus hatched from cysts. Thirteen freeze-dried cyanobacterial samples from freshwaters of Hungary, Germany and Brazil, and strains from the culture collection of the University of Helsinki were tested and analyzed for microcystins and anatoxin a by high performance liquid chromatography and in some cases, the enzyme-linked immunosorbent assay test. The samples were examined by a double blind approach. The Thamnotoxkit test reacts to pure microcystins, neurotoxins and crude extracts of cyanobacteria. It can be concluded that the Thamnotox test is an alternative simple, cost-effective method that may replace the mouse bioassay used previously for determination of cyanobacterial toxicity.