Cyanobacterial Toxins Detected by Thamnotoxkit (a Double Blind Experiment)
Abstract:
The Thamnotoxkit FTM was evaluated for detecting cyanobacterial toxins compared with the rat hepatocyte test and mouse test. This test kit is a 24-hour biosassay using larvae of the freshwater anostracan crustaceanThamnocephalus platyurus hatched from cysts. Thirteen freeze-dried cyanobacterial samples from freshwaters of Hungary, Germany and Brazil, and strains from the culture collection of the University of Helsinki were tested and analyzed for microcystins and anatoxin a by high performance liquid chromatography and in some cases, the enzyme-linked immunosorbent assay test. The samples were examined by a double blind approach. The Thamnotoxkit test reacts to pure microcystins, neurotoxins and crude extracts of cyanobacteria. It can be concluded that the Thamnotox test is an alternative simple, cost-effective method that may replace the mouse bioassay used previously for determination of cyanobacterial toxicity.
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