RANGE OF DETECTION: 40 to 500 ppb
SDI Carbaryl RaPID AssayÒ kit and Sample Diluent. SDI RaPID PrepÔ Soil Collection Kit (optional). SDI RaPID PrepÔ Extraction Kit: Type II (extraction solution, optional) Reagents: Methanol (pesticide grade), distilled or deionized water. Equipment: plastic bottles (polypropylene or high density polyethylene, 50 or 60 mL) with plastic lined screw caps, filtration apparatus (funnel and filter paper or syringe/filter) or centrifuge, equipment/tools for grinding and mixing soil, serological pipets, 25 mL graduated cylinder.
Allow frozen samples to thaw. Break up large (> 1 cm dia.) soil aggregates and mix sample thoroughly to uniformity with a mixer, mortar and pestle or by hand with a spatula or spoon. For moist samples, soil may be air dried at room temperature by spreading soil in a thin layer for up to 24 hours prior to mixing.
Add 20 mL of 3:1 methanol/water (3 parts methanol plus 1 part water) to 10 g of soil in PROCEDURE soil collection tube. Shake vigorously for 1 min. Allow particles to settle for approximately 5 minutes. If necessary to clarify, filter or centrifuge at least 0.5 mL of the extract.
Dilute the clarified extract 1:50 with the Sample Diluent. For example, measure 4.9 mL of Sample Diluent with a serological pipet into a glass test tube, then add 100 ul of extract using a precision microliter pipet and mix thoroughly. Analyze the diluted extract as the 'sample' according to package insert of the RaPID Assay.