Detection of carbendazim in grapes matrix grapes

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ANALYTE: Carbendazim, a metabolite of benomyl

RANGE OF DETECTION: 25 to 500 ppb

MATERIALS:
Benomyl/Carbendazim RaPID AssayÒ kit and Sample Diluent Food Prep Kit Reagents: acetone (pesticide grade) Equipment: homogenizer or blender with acetone resistant container cup, 50 mL polypropylene conical tubes, 13 x 100 mm glass test tubes, serological pipets, micropipette (50mL), vortex mixer, centrifuge (optional), scale or balance, nitrogen gas.

SAMPLE PREPARATION: Chop the grape sample to be analyzed coarsely by hand or in a food processor.

EXTRACTION PROCEDURE:
With homogenizer: Add 20 mL of acetone to 10 grams of chopped grape sample in a 50 mL conical tube. Homogenize the sample thoroughly to disrupt fruit tissues. With blender: Weigh out an amount of chopped sample that provides a representative subsample of the grapes to be analyzed. Add the weighed sample to a blender with an acetone resistant cup. Add a volume of acetone (in mL) equal to twice the weight (in grams) of the grapes, e.g. 50 g grapes plus 100 mL acetone. Blend the mixture thoroughly to disrupt fruit tissues. Transfer approximately 25 mL of the mixture to a 50 mL conical tube. Allow large particulates in the homogenate to settle to the bottom of the conical tube (about five minutes) or centrifuge gently if desired. Transfer approximately 4 mL of the acetone extract (upper layer) to an Extract Cleanup Reagent Tube from the Food Prep Kit. Vortex the tube for 15 seconds. Using the scoop provided in the kit, add one scoopful of Salt Reagent to the tube. Cap the tube and vortex to mix thoroughly. Allow the aqueous and acetone phases to separate for about 5 minutes.

ANALYSIS:
Carefully transfer 50 mL of the acetone phase (top layer) to a glass test tube and evaporate to dryness under nitrogen. Redissolve the residue in exactly 2.5 mL of the Sample Diluent. Analyze the diluted extract as the 'sample' according to package insert of the RaPID Assay.

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