ANALYTE: Carbendazim, a metabolite of benomyl
RANGE OF DETECTION: 25 to 500 ppb
Benomyl/Carbendazim RaPID AssayÒ kit and Sample Diluent Reagents: acetone (pesticide grade), sodium chloride. Equipment: blender with acetone resistant container cup, 50 mL polypropylene conical tubes, 15 mL polypropylene centrifuge tubes with screw caps, 13 x 100 mm glass test tubes, serological pipets, vortex mixer, scale or balance.
Add 100 mL of acetone to 50 grams of potato in the container cup and blend 2 minutes on high speed. Transfer approximately 50 mL of the blended mixture to a 50 mL conical tube and allow the solids to settle for about 5 minutes. Transfer about 4 mL of the extract (upper layer) to a 15 mL centrifuge tube and add 1/4 teaspoon (or approx. 2.0 g) of sodium chloride. Cap the tube and vortex to mix thoroughly. Allow the aqueous and acetone phases to separate (about 5 minutes).
Transfer 100 mL of the acetone phase (top layer) to a glass test tube and evaporate to dryness under nitrogen. Redissolve the residue in exactly 5 mL of the Sample Diluent. Analyze the diluted extract as the 'sample' according to package insert of the RaPID Assay.