RANGE OF DETECTION: 10 to 500 ppb
Carbofuran RaPID AssayÒ kit and Sample Diluent. Reagents: methanol (pesticide grade), distilled or deionized water. Equipment: reciprocating shaker, plastic bottles (polypropylene or high density polyethylene, 50 or 60 mL) with plastic lined screw caps, filtration apparatus (funnel and filter paper or syringe/filter) or centrifuge, equipment/tools for grinding and mixing soil, serological pipets.
Allow frozen samples to thaw. Break up large (> 1 cm dia.) soil aggregates and mix sample thoroughly to uniformity with a mixer, mortar and pestle or by hand with a spatula or spoon. For moist samples, soil may be air dried at room temperature by spreading soil in a thin layer for up to 24 hours prior to mixing.
Useful results can be obtained with extraction times as short as five minutes, depending on the objective of the study, especially with large sample loads and when time and cost constraints are critical. The following protocol is recommended when assessing ppb levels and when extracting most of the pesticide for analysis is required.. Add 20 mL of 3:1 methanol/water (3 parts methanol plus 1 part water) to 10 g of soil in the plastic bottle. Position the sample bottle on its side and shake vigorously on reciprocating shaker at high speed (200 cycles/min.) for 30 minutes. Allow sample to sit 16 to 24 hours. Then shake sample vigorously again for 30 minutes. Allow soil particles to settle for approximately 15 minutes. If necessary to clarify, filter or centrifuge at least 0.5 mL of the extract.
Dilute the clarified extract 1:50 with the Sample Diluent. For example, measure 4.9 mL of Sample Diluent with a serological pipet into a glass test tube, then add 0.1 mL of extract using a precision microliter pipet and mix thoroughly. Analyze the diluted extract as the 'sample' according to package insert of the RaPID Assay.