Detection of DNA sequences of bacteria using a direct electrochemi-luminescence method

0
- By: , ,

Courtesy of

Real-time detection of microbiological contamination in water is a challenging issue in comparison to the state-of-the-art re-quired minimum test-duration of 48 to 72 hours. Pathogen bac-teria in drinking water, like Escherichia Coli, can be detected by identification of their DNA. First, the specific sequences of the DNA have to be selected by hybridization with a corre-sponding probe. The hybridized product can then be detected. The identification is realized by using the following process:
– Extraction of the DNA of bacteria (all types)
– Denaturation of DNA
– Labeling of single strands
– Selection of DNA by hybridization with a complementary sequence of oligonucleotides of the target bacteria
– Detection and quantification by using the electro-chemilu-minescence property of the label.

Customer comments

No comments were found for Detection of DNA sequences of bacteria using a direct electrochemi-luminescence method. Be the first to comment!