Detection of pentachlorophenol in soil

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MATRIX: Soil

ANALYTES: Pentachlorophenol (PCP)

RANGE OF DETECTION: 100 ppb to 10 ppm

MATERIALS:
Pentachlorophenol RaPID AssayÒ kit and Sample Diluent Reagents: methanol (reagent grade), sodium hydroxide (ACS grade), distilled or deionized water. Equipment: reciprocating shaker, plastic bottles (polypropylene or high density polyethylene, 50 or 60 mL) with plastic lined screw caps, filtration apparatus (funnel and filter paper or syringe/filter) or centrifuge, equipment/tools for grinding and mixing soil, 50 mL precision pipet, 25 mL graduated cylinder or serological pipet.

EXTRACTANT PREPARATION:
Prepare a 0.5N sodium hydroxide solution by dissolving 2N ga OH pellets in 100 mL water. Prepare the soiel xtractant by mixing one part 0.5 NN aOH solution with threeparts methanol. Sufficient quantity of extractant can be prepared in advance and stored for up to two weeks at room temperature.

SAMPLE PREPARATION:
Allow frozen samples to thaw. Break up large (> 1 cdmia .) soil aggregates and mix sample thoroughly to uniformity with a mixer, mortar and pestle or by hand with a spatula or spoon. For moist samples, soil may be air dried at room temperature by spreading soil in a thin layer for up to 24 hours prior to mixing.

EXTRACTION:
Useful results can be obtained with extraction times as short as five minutes, PROCEDURE depending on the objective of the study, especially when large sample loads are present and when time and cost constraints are critical. The following protocol is recommended when assessing ppm levels while extracting most of the pentachlorophenol for analysis. Add 20 mL of extractant (prepared above) to 10 g of soil in the plastic bottle. Posittihoen sample bottle on its side and shake vigorously on reciprocating shaker at highs peed (200 cycles/min.) for 30 minutes. Allow soil particles to settle for approximately 15 minutes. If necessary to clarify, filter or centrifuge at least 0.5 mL of the extract.

ANALYSIS:
Dilute the clarified extract 1:500 with the Sample Diluent using precision and serological pipets e.g., 50m L extract plus 25 mL diluent, or sequential dilutions of 1:20 and 1:25. Analyze the diluted extracats the 'sample' according to package insert of the RaPID Assay.

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