Detection of procymidone in grapes


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MATRIX: Grapes

ANALYTE: Procymidone

RANGE OF DETECTION: 0.4 to 20 ppm

Procymidone RaPID Assay Kit and Sample Diluent Reagents: acetone (pesticide grade), sodium chloride. Equipment: blender with acetone resistant container cup, 50 mL conical tubes, 15 mL polypropylene centrifuge tubes with screw caps, serological pipets, centrifuge, scale or balance.

Add 100 mL of acetone to 50 grams of grapes in the container cup and blend 2 on high speed. Transfer approximately 50 mL of the blended mixture to a 50 mL conical tube and allow the solids to settle for about 10 minutes. Transfer about 4 mL of the extract (upper layer) to a 15 mL centrifuge tube, and add 1/4 teaspoon (or approx. 2.0 g) of sodium chloride. Cap the tube and vortex to mix thoroughly. Centrifuge at 1000 x g for 5 minutes to separate the aqueous and acetone phases.

Dilute the acetone phase (upper layer) 1:100 in Sample Diluent using serological pipets (e.g. 0.1 mL acetone extract plus 9.9 mL diluent). Analyze the diluted extract as the 'sample' according to package insert of the Procymidone RaPID Assay.

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