Development of a homologous protein carrier system for heterologous protein production in Myceliophthora thermophila

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Introduction

Filamentous fungi can produce and secrete large quantities of extracellular enzymes. Species such as Aspergillus niger, Aspergillus oryzae, Trichoderma reesei and also Myceliophthoro thermophila CI are used in industry as work horses for enzyme production. High yields of homologous enzymes can be readily obtained using these micro-organisms. On the contrary, heterologous proteins are often produced at low levels. Dyadic uses the fungus Myceliophthoro thermophila CI as a platform for the hyper production of a broad variety of proteins, mainly enzymes. Here we describe the development of a CI homologous protein carrier system for improved heterologous protein production.

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