Does crustacean ethoxyresorufin O‐deethylase activity vary during the molting cycle?
We report herein whether microsomal EROD activity in the hepatopancreas fluctuates during the molting cycle of the fiddler crab, Uca pugilator. Results showed that microsomal EROD activity varies significantly during the molting cycle, with the lowest enzymatic activity occurring in late premolt stage. These results clearly show that crustacean EROD activity is influenced by the molting physiology, suggesting that when using crustacean EROD assays in evaluating pollution, only individuals from the same molt stage should be used. We propose that the high level of EROD activity in postmolt and intermolt stages is an additional mechanism used by crustaceans to prevent any untimely rise in ecdysteroid levels. Environ Toxicol Chem © 2013 SETAC