Keywords: calcium phosphate, CaP, fibronectin adsorption, FN, protein adsorption, nanoscale topography, sputter deposition, thin films, nanomaterials, thin film surfaces
Effect of nanoscale topography on fibronectin adsorption to sputter deposited calcium phosphate thin films
Fibronectin (FN) adsorption to Calcium Phosphate (CaP) thin film surfaces with topographical features in the submicron to nanometre range has been investigated. CaP thin films have been produced by Radio-Frequency (RF) magnetron sputter deposition and a sputtered Titanium (Ti) interlayer was used to create substrates with nanoscale surface features. Detailed X-ray Photoelectron Spectroscopy (XPS) analysis of the FN ad-layers indicated differences in the nature of the adsorption on the main surface types. Binding studies revealed that the FN had a stronger affinity for the CaP on sputtered Ti. These data suggest that nanoscale topography can influence FN adsorption on CaP thin film surfaces.