John Wiley & Sons, Ltd.

Effect of straw leachates from Cry1Ca‐expressing transgenic rice on the growth of Chlorella pyrenoidosa

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Because of the prevalence of algae in rice paddy fields, they will be exposed to Bacillus thurigiensis (Bt) proteins released from Bt protein‐expressing genetically engineered rice. To assess the effects of leachates extracted from Cry1Ca‐expressing transgenic rice (T1C‐19) straw on the microalga Chlorella pyrenoidosa, we added purified Cry1Ca (10, 100, 1000 µg/L) and five concentrations of diluted extracts (5, 10, 20, 40, 80%) from T1C‐19 and the non‐transformed control strain MH63 into the medium of C. pyrenoidosa. We found that (1) the growth curves of C. pyrenoidosa treated with purified Cry1Ca were overlapping with the medium control; (2) the order of C. pyrenoidosa growth rates for the T1C‐19 leachate concentrations was 5% > 10% > 20% > control > 40% > 80% and for the MH63 concentrations was 5% > 10% > control > 20% > 40% > 80%, but there were no statistical differences between the 20% T1C‐19 or 20% MH63 leachate treatment and the medium control on day 8; (3) after 7 days of culture, Cry1Ca could be detected in C. pyrenoidosa treated with different concentrations of T1C‐19 leachate. Our results demonstrated that Cry1Ca protein released from T1C‐19 rice can be absorbed into C. pyrenoidosa, but that purified Cry1Ca and leachates from T1C‐19 rice have no obvious adverse effects on the growth of C. pyrenoidosa. Environ Toxicol Chem © 2014 SETAC

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