John Wiley & Sons, Ltd.

Effects of landfill leachate treatment on hepatopancreas of Armadillidium vulgare (Crustacea, Isopoda)

The major environmental impact to the landfill is emission of pollutants via the leachate and gas pathway.The hepatopancreas of the terrestrial isopod Armadillidium vulgare (Isopoda, Crustacea, Latreille 1804) takes an important role in bioaccumulation of contaminants, such as heavy metals. To evaluate the effects of landfill leachate treatment, two different approaches are here applied: 1) the detection of accumulation of trace elements (As, Cd, Cr, Cu, Sb, Zn, Pb, Ni, V) in hepatopancreatic cells and 2) the evaluation of biological effect of contaminants on fresh hepatopancreatic cells, by flow cytometric analyses. The presence of two different cell types (S and B cells‐in agreement with literature data based on morphological examinations) is detected for the first time by Flow Cytometry, highlighting also their different response to stress stimuli. In particular B cells appear more sensible to landfill leachate treatment, being more damaged at short term, while S cells seem more adaptive. Furthermore S cells could represent a pool from which they are able to differentiate in B cells. These findings were also confirmed by PCA analyses underlining S SYBR Green I Bright cells correlate with specific chemicals (Ca, Cu, Co) confirming their resistance to stress stimuli and suggesting that the decrease of specific cell types may prime other elements to replace them, in a homeostasis preservation framework. Environ Toxicol Chem © 2013 SETAC

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