John Wiley & Sons, Ltd.

Effects of the anti‐microbial contaminant triclocarban, and co‐exposure with the androgen 17β‐trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas)

0
Triclocarban (TCC) is an antimicrobial agent routinely detected in surface waters which has been hypothesized to interact with the vertebrate endocrine system. The present study examined the effects of TCC alone and in combination with the model endocrine disruptor 17β‐trenbolone (TRB) on fish reproductive function. Adult Pimephales promelas were continuously exposed to either 1 or 5 µg TCC/L, 0.5 µg TRB/L or a mixture (MIX) of 5 µg TCC and 0.5 µg TRB/L for 22 d and a variety of reproductive and endocrine‐related endpoints were examined. Cumulative fecundity was significantly reduced in fathead minnows exposed to TRB, MIX or 5 µg TCC/L. Exposure to 1 µg TCC/L had no effect on reproduction. In general both TRB and MIX treatments caused similar physiological effects, evoking significant reductions in female plasma vitellogenin, estradiol, and testosterone, and significant increases in male plasma estradiol. Based on analysis of the ovarian transcriptome, there were potential pathway impacts that were common to both TRB and TCC‐containing treatment groups. However, in most cases, those pathways were more plausibly linked to differences in reproductive status than to androgen‐specific functions. Overall, TCC was reproductively toxic to fish at concentrations at or near those that have been measured in surface water. There was little evidence that TCC elicits reproductive toxicity through a specific mode of endocrine or reproductive action, nor that it could augment the androgenic effects of TRB. Nonetheless, the relatively small margin of safety between some measured environmental concentrations and effect concentrations suggest concern is warranted. This article is protected by copyright. All rights reserved

Customer comments

No comments were found for Effects of the anti‐microbial contaminant triclocarban, and co‐exposure with the androgen 17β‐trenbolone, on reproductive function and ovarian transcriptome of the fathead minnow (Pimephales promelas). Be the first to comment!