Enumeration of salmonella bacteria in food and feed samples by real-time pcr for quantitative microbial risk assessment

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Quantitative microbial risk assessment (QMRA) is an important approach for food safety in which risk and factors that influence food safety are identified. The goal is to provide an estimate of the level of illness that a pathogen can cause in a given population (13). For QMRA, there is a need for microbiological methods that generate quantitative data. Furthermore, the sample preparation methods preceding the analytical method itself (e.g., PCR) need to be able to produce quantitative results.

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