A 1,026-bp open reading frame sharing significant similarity with queA, which encodes a predicted S-adenosylmethionine:tRNA ribosyltransferase-isomerase responsible for queosine modification of tRNAs, was found immediately 5' of the gene for the transcriptional activator (ArcR) of the arginine deiminase system (ADS) operon of Streptococcus gordonii. The role of QueA in bacterial physiology is enigmatic, but loss of QueA has been shown to compromise stationary-phase survival or virulence in certain enteric bacteria. Interestingly, S. gordonii appears to be unique among ADS-positive bacteria in the linkage of queA with the ADS genes. A putative 70 promoter (pqueA; TTGCCA-N21-TATAAT) was mapped 5' of queA by primer extension, and queA and arcR were shown to be cotranscribed. The expression from pqueA was found to be constitutive under all conditions tested, but the expression of parcA, which drives the expression of the arc structural genes, was enhanced in stationary phase and could be induced by low pH and arginine. QueA and CcpA acted repressively on arc transcription, but neither QueA-deficient strains nor CcpA-deficient strains showed significant differences in arginine deiminase enzyme activities compared with the wild-type strain. The growth rate of a QueA-deficient strain did not differ significantly from that of the parental strain, but the QueA-deficient strain did not compete well with the wild-type during serial passage. In addition to the finding that ADS expression can be regulated separately by growth phase and pH, a significant linkage between the ADS, translational efficiency modulated by QueA, and post-exponential-phase survival of S. gordonii was found.
- American Society for Microbiology
- Environmental and growth phase regulation of the streptococcus ...
Are Universal Wastes DOT Regulated?
The answer is yes, no, and maybe. Under the DOT regulations, hazardous wastes are regulated in all modes (ground, air, rail, vessel) of transportation. EPA has classified specific hazardous waste as Universal Waste to streamline the process and encourage recycling of these materials (see 40 CFR 273). EPA has specifically addressed the DOT requirements for these waste at 40 CFR 273.52 as follows: § 273.52 – Waste management.(a) A universal waste transporter must comply with all applicable U.S....
Stolthaven Terminals Environmental Reporting - Case Study
Learn how Stolthaven Terminals uses webEI to manage TCEQ and EPA requirements while improving reporting. Tim Friday discusses how the implementation of regAction’s webEI software and support services reduced the challenges faced with collecting and organizing the reporting data. Its flexibility and ease of use helps Stolthaven maintain the company’s quality standards and meet environmental regulations. Stolthaven is currently utilizing programs from regAction to track and manage Waste, Emissions...
Environmental taxation in the right place can increase business productivity
Industry has traditionally claimed that strict environmental regulation has a negative effect on its competitiveness. However, a recent theory proposes it may actually increase productivity and innovation. This study used a large database of inter-sectoral transactions to investigate the effect of environmental taxation on manufacturing businesses across Europe. The findings show that environmental regulation can increase innovation and productivity. Environmental regulation is essential to limit the effects of...
Heterogeneity of the environmental regulation of industrial wastewater: European wineries
The European legislation of the pollution of industrial wastewater shows a high degree of heterogeneity. This fact implies that there is a market failure with relevant consequences. Within the European Union, each Member State performs a specific transposition of the Water Framework Directive 2000/60. The member states introduce different sanitation fees to correct water pollution. In this paper, the case of the European wine industry is analyzed. It studies the sanitation fees of the five major wine producing...
Noise at Work Regulations: 10 Years On
Doesn’t time fly? It’s already been 10 years since Control of Noise at Work Regulations were introduced in the UK. But have you been following the legislation correctly? Here’s an overview of what the Noise at Work Regulations mean and some common mistakes you should be avoiding. Considering that legislation to limit noise at work has been around for at least a couple of decades, I find it staggering that we currently have around 18,000 people in the UK suffer deafness, tinnitus or other...