Reliable prediction of time-varying pollutant loads in combined sewer systems during storm periods can aid better management of the release of pollution into natural environments as well as enhancing storage tank design. Better understanding of the behaviour of sewer sediments is crucial for the development of models that adequately describe the transport of in-sewer solids and accurately predict the changes in pollutant concentration within combined sewers during storm events. This paper reports on the results of a test programme to examine the erosion of highly organic sewer sediment under the application of time-varying shear stress. The tests were carried out with and without supplying oxygen, and varying simulated dry-weather periods. The aim was to investigate the behaviour of real in-sewer sediment with a high organic content (around 80%) in an attempt to improve prediction of the transport rates under the particular Mediterranean conditions of long dry-period/build-up and intense rainfall/wash-off, and understand how this environment affects the erosional resistance and subsequent sediment release. Results have been compared with previous work on lower organic content sewer sediments and artificial organic sediment.