Keywords: ionising radiation, radiation workers, low dose, biodosimetry, QPCR, Bax, Bcl–2, apoptosis, gene expression, apoptotic genes, low radiation, DNA damage, biomarkers, radiation exposure
Expression of apoptotic genes can distinguish radiation workers from normal population
Ionising radiation can be produce different forms of DNA damage on living cells. Changes in biomarkers due to exposure to ionising radiation can be an appropriate tool for the immediate recognition of individuals exposed to radiation after a radiological accident or nuclear disaster. QPCR technique, as an approved method, can be used for analysing the expression levels of sensitive genes following radiation exposure. Gene expression studies were performed on a group of 36 radiation workers and 36 matched–normal people without a history of radiation exposure. Mean gene expression values for control and irradiated groups were compared. Mean gene expression values for control and irradiated groups were compared. There were significant differences between the mean gene expressions of Bax and Bcl–2 (p<0.05), in normal and irradiated groups. The logistic regression model is able to correctly identify 77.8% of irradiated samples (specificity) and 75.0% of normal samples (sensitivity).