Fast LC-MS-MS Quantitation of N-Methyl Carbamate Pesticides in Food using Bond Elut® Plexa™ and Pursuit® XRs C18

0
- By: , , ,

Courtesy of

A fast quantitation method for the analysis of N-Methyl Carbamate pesticide residues in vegetables and grains has been developed with Bond Elut Plexa clean-up, Pursuit XRs C18 columns, and 320 LC-MSMS. High recoveries were obtained for most carbamates screened in all matrices tested at 1 ppb levels. Good linearity of the calibration
curves was observed for all analytes, over the range from 1 ppb to 200 ppb levels, with r2 greater than 0.99.

N-Methyl Carbamate pesticides are widely used as insecticides or fungicides. Even when applied in accordance with Good Agricultural Practices (GAP), they can leave residues, which can be detrimental to food safety (1). There is a growing pressure from governmental agencies and private companies to improve the analytical performance
in pesticide residue analysis, making it necessary to increase the efficiency and lower the cost and time of these analyses (2). Carbamates are currently quantitated using HPLC with fluorescence detection. Since HPLC-fluorescence detection does not provide the required specificity for determining pesticide residues, tandem LC-MS methods have recently been adopted. The main advantage offered by tandem LC-MS is that the chromatography data
acquisition is much faster than that obtained from HPLC-fluorescence. We have adapted to an efficient, and cost-effective acetonitrile-based liquid-liquid extraction method and Bond Elut Plexa clean-up, which yields good recoveries for a number of food matrices, tested.

Experimental
Matrices tested: Leafy vegetables like lettuce, and grains like rice and wheat flour were selected for screening carbamate pesticides. Carbamate pesticides screened: Aldicarb, Aldicarb Sulfone, Aldicarb Sulfoxide, Carbaryl,
Carbofuran, 3-Hydroxycarbofuran, Methomyl, Oxamyl, Methiocarb, Propoxur, and BDMC (4-Bromo-3,5-dimethylphenyl-N-methylcarbamate, Internal Standard).

Customer comments

No comments were found for Fast LC-MS-MS Quantitation of N-Methyl Carbamate Pesticides in Food using Bond Elut® Plexa™ and Pursuit® XRs C18. Be the first to comment!