Keywords: platinum nanowires, nano–biosensors, glucose biosensors, diabetic blood, clinic study, nanotechnology, glucose measurement, diabetes diagnosis
Glucose biosensor based on platinum nanowires: a clinical study
We describe the process of modification of the platinum (Pt) nanowire surface and subsequent immobilisation of enzyme glucose oxidase (GOx) on Pt nanowires (PtNWs) for direct glucose measurement. A cyclic voltammetry technique was used to measure electrochemical oxidation between GOx and glucose. PtNW arrays were first fabricated and then functionalised with cysteamine, which in turn acted as a chemical linkage for the enzyme conjugation. Afterward, the enzyme modified PtNWs arrays were subjected to the model test to check the effect of the process modification. The cyclic voltammograms of GOx immobilised Pt chips in PBS (1M, pH of 7) showed the most stable result. The enzymatic modified PtNW electrodes were then successfully determined glucose in diabetic blood samples with the concentrations from 125 µM to 16.5 mM, a deviation of ± 3%. The obtained results clearly indicate the potential of a next–generation of glucose sensor based on PtNW in application diabetic diagnosing.