In response to the need for methods that can rapidly detect potentially virulent Bacillus anthracis spores, we developed a two-color flow cytometric assay capable of simultaneously identifying B. anthracis spores and the presence of spore-associated protective antigen, a virulence marker for strains harboring the pXO1 plasmid.
- American Society for Microbiology
- Identification and characterization of bacillus anthracis spores ...
Heavy metal tolerance and removal potential in mixed-species biofilm
The aim of the study was to examine heavy metal tolerance (Cd2+, Zn2+, Ni2+ and Cu2+) of single- and mixed-species biofilms (Rhodotorula mucilaginosa and Escherichia coli) and to determine metal removal efficiency (Cd2+, Zn2+, Ni2+, Cu2+, Pb2+ and Hg2+). Metal tolerance was quantified by crystal violet assay and results were confirmed by fluorescence microscopy. Metal removal efficiency was determined by batch biosorption assay. The tolerance of the mixed-species biofilm was higher than the single-species biofilms....
Robust on-line monitoring assay - Case Study
Alkaline Phosphatase Enzyme Activity Measurement as a Robust on-line Monitoring Assay for Water Quality Assessment. Zina Al-Saar 4th year Intern student Avans University of Applied Science - ATGM Department Joep Appels - Supervisor and Managing Director microLAN on line Bio-monitoring System. Many heterotrophic microorganisms maintain their growth by producing enzymes in order to provide low molecular weight inorganic compounds. These compounds could be up taken into the cells from high molecular weight...
Nutrient recovery from airplane wastewater: composition, treatment and ecotoxicological assay
For the 2014 World Cup and the 2016 Olympic Games, Brazil has expanded its airport infrastructure. This will lead to an increase in wastewater generation from aircrafts. This wastewater is traditionally taken from the aircrafts and disposed in the public sewage collection system. However, this residual water may have a different composition than the usual sanitary sewage. Therefore, it is important to study an alternative to treat this kind of wastewater. Thus, the objective of this study was to characterize and...
Effects of drinking desalinated seawater on cell viability and proliferation
Desalination of seawater is becoming an important means to address the increasing scarcity of freshwater resources in the world. Seawater has been used as drinking water in the health, food, and medical fields and various beneficial effects have been suggested, although not confirmed. Given the presence of 63 minerals and trace elements in drinking desalinated seawater (63 DSW), we evaluated their effects on the behavior of tumorigenic and nontumorigenic cells through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl...
Online Total Bacteria Monitor compared with ATP and flow cytometry - Case Study
Alkaline Phosphatase Enzyme Activity Measurement as a Robust on-line Monitoring Assay for Water Quality Assessment. Micro organisms maintain their growth by producing enzymes in order to provide low molecular weight inorganic compounds. These compounds could be taken up into the cells from high molecular weight organic compounds which cannot be directly utilized by bacteria. Microbial enzymes are, therefore, directly related to the concentrations and ratios of limiting nutrients, and may be sensitive indicators...